B-NDG Kit W41 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Immunodeficient Mice/Rats

Immunodeficient (B-NDG) Mice

Text

B-NDG Kit W41 mice

Strain Name	NOD.CB17-Prkdc^scid Il2rg^tm1Bcgen Kit^{tm1(V831M)Bcgen}/Bcgen	Common Name	B-NDG Kit W41 mice
Background	B-NDG	Catalog number	112914
Aliases	Human: PBT, SCFR, C-Kit, CD117, MASTC
NCBI Gene ID	3815

Description

C-Kit is expressed in various cells within the body, and it is significantly expressed in stem cells, progenitor cells, and other cells with self-renewal capabilities. In normal bone marrow, c-Kit is expressed by hematopoietic stem cells and plays a crucial role in self-renewal and differentiation into various blood cells. After binding with its ligand SCF, c-Kit undergoes autophosphorylation, thereby activating downstream signaling pathways and participating in cellular processes such as cell proliferation, differentiation, survival, adhesion, motility, and angiogenesis.

Gene editing strategy: The 831 acid amino Val of Kit gene was mutated as Met in B-NDG Kit W41 mice.

mRNA expression analysis: Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.

Application: This product can accept human hematopoietic stem cell transplantation without irradiation, making it suitable for research in the fields of blood and immunity.

Mechanism of action

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After the SCF dimer binds to the extracellular structural domain of CD117, CD117 undergoes autophosphorylation and is activated, subsequently triggering a variety of signal transduction pathways：

JAK/STAT Pathway
RAS/MAP Kinase Pathway
PI3-Kinase/Akt Pathway
SRC Family Kinase Pathways
PLCγ Pathway

Targeting strategy

Gene targeting strategy for B-NDG Kit W41 mice.
The 831 acid amino Val of Kit gene was mutated as Met in B-NDG Kit W41 mice.

Model validation

√: The experiment is planned.
NA: The experiment is not currently planned.
According to the characteristics and applications of the products, you can add experiments in the table by yourself.

mRNA expression analysis

Strain specific analysis of Kit mRNA expression in B-NDG mice and B-NDG Kit W41 mice by RT-PCR. Spleen and lung RNA were isolated from B-NDG mice (+/+) and heterozygous B-NDG Kit W41 mice (Mut/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Kit primers. Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.

Summary

mRNA expression analysis:
Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.